Figure 1.
Development of chronic colitis is accompanied by enhanced myelopoiesis and increase in peripheral granulocyte numbers. A. Blinded histopathological scores of colons obtained from CD45RBhigh→RAG−/−, CD45RBlow→RAG−/− and CD45RBhigh+CD45RBlow→RAG−/− mice for at least 11 animals per group from at least 2 independently conducted experiments. B. Total leukocyte numbers in peripheral blood of mice from the different groups. Blood leukocyte counts in mice were determined as described in the Materials and Methods section. C. Total numbers of PMNs/PMN-ring cells in peripheral blood in mice. Representative images of peripheral blood cytospins from wild-type (WT) (D) and colitic CD45RBhigh→RAG−/− mice (E) showing dramatic increases in peripheral blood granulocytes. Serum from 7-11 individual mice per group was analyzed for G-CSF (F) and IL-17 (G). Data in all graphs shown represent the mean±SEM. Significance between groups is indicated by asterisks: * indicates p<0.05, ** indicates p<0.01 and *** indicates p<0.001.