FIGURE 5.
Subcellular localization of recombinant wild type or mutant talin J-DsRed fragments (talin J wt, talin J K2085D, and talin J K2089D). CHO cells expressing αIIbβ3wt were transiently transfected with cDNAs encoding the different talin J fragments, as described under “Experimental Procedures,” and plated for 2 h at 37 °C on FIB. Cells were subjected to immunofluorescence staining using P37 as anti-β3 mAb (green). The subcellular localization of talin J fragments fused to DsRed was compared with the localization of β3 integrins. The bar equals 10 μm.