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. Author manuscript; available in PMC: 2012 Aug 1.

Published in final edited form as: J Invest Dermatol. 2011 Oct 13;132(2):458–465. doi: 10.1038/jid.2011.324

The density of mast cells (a) and granule remnants (b) were determined in the dermis adjacent to wounds in E15 ( ) or E18 (■) fetal skin using toluidine blue-stained sections. Values for unwounded control skin (ctrl) are included for reference. Bars represent the mean ± SEM (n=3–9 fetuses per group; *p<0.05, **p<0.01, ***p<0.001). Representative photomicrographs of toluidine blue-stained E15 (c) and E18 (d) skin harvested at 5 hours post-wounding are shown. Scale bars = 50 μm and dotted lines mark the dermal-epidermal junction. Arrows indicate mast cells ( ) and granule remnants ( ).

Inline graphic — The density of mast cells (a) and granule remnants (b) were determined in the dermis adjacent to wounds in E15 ( ) or E18 (■) fetal skin using toluidine blue-stained sections. Values for unwounded control skin (ctrl) are included for reference. Bars represent the mean ± SEM (n=3–9 fetuses per group; *p<0.05, **p<0.01, ***p<0.001). Representative photomicrographs of toluidine blue-stained E15 (c) and E18 (d) skin harvested at 5 hours post-wounding are shown. Scale bars = 50 μm and dotted lines mark the dermal-epidermal junction. Arrows indicate mast cells ( ) and granule remnants ( ).