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. 2011 Jun 10;22(2):403–416. doi: 10.1093/cercor/bhr122

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Figure 6. — Increased number of Dbx1-derived CP transient neurons migrating in the developing pallium of Gsx2 mutants. (A–D) Immunohistochemistry for GFP and Tbr1 shows an increase in the number of GFP⁺ cells migrating tangentially into the pallium of E13.5 Dbx1^CRE/+;CagCat-EGFP;Gsx2^RA/RA mutant animals (B,D) compared with Dbx1^CRE/+;CagCat-EGFP;Gsx2^+/+ control littermates (A,C). A higher number of Dbx1-derived GFP⁺Tbr1⁺ neurons is detected in the pallium of mutants compared with controls (C,D magnification of dashed boxes in A,B, respectively). (E–H) Immunohistochemistry for GFP and Mef2c on E18.5 Dbx1^CRE/+;CagCat-EGFP;Gsx2^RA/RA mutant animals (G,H) compared with Dbx1^CRE/+;CagCat-EGFP;Gsx2^+/+ control littermates (E,F) confirms an increment in the number of GFP⁺Mef2c⁺ neurons in the CP at dorsolateral levels. (I) Graph represents the total number of GFP⁺Tbr1⁺ and GFP⁺Mef2c⁺ neurons in the CP of coronal sections of mutant and control cortices at E18.5, corresponding to the number of Dbx1-derived CP transient neurons, as well as the total number of GFP⁺Tbr1⁺ neurons in the MZ, corresponding to Dbx1-derived CR cells. These results confirm a strong increment in the number of Dbx1-derived glutamatergic neurons in mutants (GFP⁺Tbr1⁺: 23 ± 2.51 neurons per sections in controls vs. 51.66 ± 8.01 in mutants, P = 0.026; GFP⁺Mef2c⁺: 25 ± 4.04 neurons per sections in controls vs. 93.66 ± 23.14 in mutants, P = 0.043) as compared with a slight increase in the number of Dbx1-derived CR cells (GFP⁺Tbr1⁺: 3.83 ± 0.16 neurons per sections in controls vs. 5.18 ± 0.49 in mutants, P = 0.060). Graphs represent means ± SEM. *P < 0.05. Scale bars: A, 100 μm; E,F, 50 μm.