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. 2011 Jun 10;22(2):403–416. doi: 10.1093/cercor/bhr122

Figure 2.

Figure 2.

Transient increase in differentiation progressively affects the entire pallium of E1-Ngn2/CRE;Dbx1DTA mutants. (A–H) Immunohistochemistry performed on E12.5 embryos shows no differences for Tbr1 (A,B) and TuJ1 (C,D) staining between control (A,C) and E1-Ngn2/CRE;Dbx1DTA (B,D) embryos at rD levels, whereas there is a strong increase for both in cL regions in mutants (F,H, black bars) compared with control littermates (E,G). Moreover, ectopic Tbr1+ and TuJ1+ cells are observed in the mutant VZ (arrowheads in F,H). (I–N) By E14.5, Tbr1 staining is increased at rD levels in mutants (J,L), whereas no differences are detected at cL levels (N) with respect to control littermates (I,K,M). Ectopic Tbr1+ neurons (arrowheads in L,N) are observed in the VZ of mutants at both levels as well as a thinning of the VZ at cL levels (black bars in M,N). K and L represent high magnifications of boxed areas in I and J, respectively. Scale bars: A,K, 20 μm; I, 100 μm.