Table 3.
Primers and probes used in this study for mRNA measurementsa
| Target mRNA | Primer or probe | Sequence (5′-3′) | Reference |
|---|---|---|---|
| PPIA | FW | CGCGTCTCCTTTGAGCTGTT_ | 13 |
| RV | CTGACACATAAACCCTGGAAT AATTC_ | ||
| TP | CAGACAAGGTCCCAAAGACAGCAGAAAATTT_ | ||
| IL-8 | FW | TGGCAGCCTTCCTGATTTCT_ | 2 |
| RV | TTAGCACTCCTTGGCAAAACTG_ | ||
| TP | CAGCTCTGTGTGAAGGT_ | ||
| TNF | FW | TCTTCTCGAACCCCGAGTGA_ | 10 |
| RV | CCTCTGATGGCACCACCAG_ | ||
| TP | TAGCCCATGTTGTAGCAAACCCTCAAGCT_ | ||
| IL-10 | FW | GTGATGCCCCAAGCTGAGA | 10 |
| RV | CACGGCCTTGCTCTTGTTTT | ||
| TP | CCAAGACCCAGACATCAAGGCGCA |
a
FW, forward primer; RV, reverse primer; TP, TaqMan probe, dually labeled with 5′ 6-carboxyfluorescein and 3′ 6-carboxytetramethylrhodamine; PPIA, peptidyl-prolyl cis-trans isomerase A; IL-8, interleukin-8; IL-10, interleukin-10; TNF, tumor necrosis factor.