FIG. 2.
M2 responses in adherent splenocytes from young and aged mice. Relative mRNA expression of (A) Arg1, (B) FIZZ1, and (C) Ym1 was quantified by real-time PCR using cDNA from adherent cells obtained from the spleens of young and aged mice. Cells were stimulated in vitro for 20 h in the presence of IL-4, LPS, or a combination of IFNγ and TNF-α. N=3–7 mice *P<0.05 compared with young of the same treatment group. All data are normalized to values from media-treated samples from young mice to highlight the baseline differences between the age groups, if any. Arg1, arginase-1; FIZZ1, Found In Inflammatory Zone 1.