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. 2012 Jan 1;23(1):163–175. doi: 10.1091/mbc.E11-05-0474

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© 2012 Marat et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Full-length connecdenn 3 exhibits GEF activity toward Rab35 but not Rab13. (A) HEK-293T cells were transfected with full-length FLAG-tagged connecdenn 3 (CD3) or were mock transfected, and cell lysates were processed for immunoprecipitation with α-FLAG antibody, followed by detection with α-FLAG antibody. (B) Immunoprecipitated connecdenn 3 or mock immunoprecipitations were used in GEF assays measured as the incorporation of [³⁵S]GTPγS onto 1.25 μM GDP-loaded Rab13 or Rab35 as a function of time. The number of picomoles of incorporated GTPγS is plotted over time. A representative plot is shown.