FIGURE 5.
Potency of inhibition of catalytic activity of MRSA PK by cis-3,4-dihyrohamacanthin B (left) and bromodeoxytopsentin (right). Potency of inhibition of MRSA PK was determined as described under “Experimental Procedures” with 10 μm P-enolpyruvate and 2 mm ADP substrates. The data are derived from one of three experiments with similar results each performed in triplicate and analyzed with Prism GraphPad software (single-site model). Error bars indicate the range of values within the single experiment shown. The final concentration of MRSA PK protein used in these studies was 15 nm.