Fig. 3.
SNT-1 functions within a signaling cascade that includes FGF and Ras. (A) SNT-1 activity is inhibited by expression of a dominant inhibitory Ras construct. 1 ng SNT-1 and 1ng dominant inhibitory Ras (dnRas) RNA were injected, as listed. (B) SNT-1 and basic FGF (bFGF) protein synergize in the mesoderm induction assay. 50 pg SNT-1 and 1 ng/mL bFGF were used, as listed. RT-PCR analysis of animal caps cultured until midgastrula stages.
