Figure 2. Differentially expressed genes in microarray for KC sorted vs. KC LCM are validated by quantitative RT-PCR.
We performed quantitative RT-PCR using mRNA from sorted KCs (n=6) and KC LCM (n=6). All data was normalized to hARP (human acidic ribosomal protein) housekeeping gene. A two-tailed student’s t-test confirmed the differential expression of all genes tested (* p < 0.05, ** p < 0.01). Error bar denotes mean +/− SD.
