TABLE I.
Primary Antibodies
| Antigen | Immunogen | Manufacturer | Dilution |
|---|---|---|---|
| GluR2/3 | Amino acids 850–862 EGYNVYGIESVKI | Chemicon, AB1506, polyclonal, raised in rabbit, affinity purified | 1:100 |
| Synaptophysin | Synaptosome preparation from rat retina | Sigma-Aldrich, S5768, clone SVP-38, monoclonal, raised in mouse, mouse IgG1 isotype | 1:50 |
| BDNF (1) | Epitope mapping within an internal region of BDNF of human origin; amino acids 128–147 | Santa Cruz Biotechnology, N-20, sc-546, polyclonal, raised in rabbit | 1:100 |
| BDNF (2) | Recombinant full-length protein (human) | Abcam, ab6201, polyclonal, raised in rabbit | 1:200 |
| NT-3 | 11-Amino-acid peptide (YAEHKSHRGEY) corresponding to the amino acid-terminal of mouse NT-3 coupled to BSA | Millipore, AB1527, polyclonal, raised in sheep | 1:100 |
| Tubulin | Microtubule derived from rat brain, reactive to neuron specific class III β-tubulin | Covance, PRB-435P, polyclonal, raised in rabbit | 1:200 |
| Tubulin (TUJ1) | Microtubule derived from rat brain, reactive to neuron specific class III β-tubulin | Covance, MMS-435P, monoclonal, raised in mouse | 1:200 |
| Peripherin | Electrophoretically pure trp-E-peripherin fusion protein containing all but the four N-terminal amino acids of rat peripherin | Chemicon, AB-1530, polyclonal, raised in rabbit | 1:4,000 |
| Peripherin | Electrophoretically pure trp-E-peripherin fusion protein containing all but the four N-terminal amino acids of rat peripherin | Chemicon, MAB-1527, monoclonal, raised in mouse | 1:4,000 |