Figure 3.
Type I and type II spiral ganglion neurons isolated in vitro from apical and basal regions showed heterogeneous immunolabeling with anti-synaptophysin antibody. a–d: Apical type I spiral ganglion neurons displayed uniform anti-β-tubulin antibody labeling (b, blue) without anti-peripherin antibody labeling (c, red) and showed heterogeneous anti-synaptophysin labeling when viewed alone (a, green) or when a and b were merged (d). e–h: Apical type II spiral ganglion neurons displayed uniform anti-β-tubulin labeling (f, blue) with robust anti-peripherin antibody labeling (g, red) and showed heterogeneous anti-synaptophysin labeling when viewed alone (e, green) or when e and f were merged (h). i,j: Frequency histograms of anti-synaptophysin antibody irradiance measurements from a single experiment; apical type I and type II neurons and basal type I and type II neurons were all fitted with single Gaussians. k: Normalized Gaussian fits for histograms shown in i and j show that average irradiance levels of both apical type I and type II neurons were greater than those obtained from both classes of basal neurons. l,m: Frequency histograms composed of anti-synaptophysin antibody irradiance measurements from six experiments; apical type I and type II neurons were well fitted with double Gaussians, and basal type I and type II neurons were well fitted with single Gaussians. n: Normalized Gaussian fits for histograms shown in l and m. Within each region, type I and type II distributions were similar, yet irradiance levels of apical neurons were consistently higher than in both classes of basal neuron. For a magenta-green version see Supporting Information Figure 2. Scale bar = 10 μm.