Table 2.
Reference Compounds and Final Assay Concentrations
| Compound | Target | After first addition (μM) | After second addition (μM) |
|---|---|---|---|
| Amlodipine | L-type calcium channel blocker | 0.09 | 0.36 |
| Carbachol | Cholinergic agonist | 2.78 | 10.84 |
| DMSO | Vehicle | 0.03% | 0.11% |
| E-4031 | hERG channel blocker | 0.09 | 0.36 |
| Isoproterenol | β-adrenergic agonist | 0.08 | 0.33 |
| Mibefradil | T-type calcium channel blocker | 0.09 | 0.36 |
| Ref1 | T-type calcium channel blocker | 0.83 | 3.25 |
| Ref2 | T-type calcium channel blocker | 0.67 | 2.60 |
| Ref3 | T-type calcium channel blocker | 0.67 | 2.60 |
| Zatebradine | Pacemaker channel blocker | 1.11 | 4.34 |
DMSO, dimethyl sulfoxide; hERG, human Ether-à-go-go related gene.