Figure 2. Greater induction of apoptosis following EGFR TKI treatment correlates with higher basal BIM expression across a panel of EGFR mutant lung cancers.
(A) The indicated EGFR mutant lung cancer cell lines were treated with (+ TKI) or without (−TKI) 1µM gefitinib (2µM CL-387,785 for H1975 cells) for 24 hours and lysates were probed with the indicated antibodies (B) BIM RNA levels were quantified by qRT-PCR, normalized to β-Actin, and plotted against the amount of apoptosis induced by the TKI (over vehicle-control) as determined by annexin FACS. Both RNA values and apoptosis are the mean of at least three experiments (see Sup. Table 1). For the linear regression analysis, the r2 value was 0.83 and P= 0.0018.