Figure 6. Biochemical and Bioinformatics Analyses Point to Differential Changes in Protein Expression According to Message Length When ifg-1 Is Attenuated.
(A) Results of quantitative mass spectrometry of protein lysates comparing adult nematodes fed on control RNAi with ifg-1 RNAi for 2 days. Results are expressed as mean ± SEM of changes in aa (amino acid) length of genes that were increased or decreased upon ifg-1 inhibition.
(B) Bioinformatic analysis of the relationship between ORF length and GO categories. Genes were separated on the basis of ORF length (bases) into five bins containing an equal number of genes. The ORF lengths for bins 1–5 are 0–522, 523–873, 874–1101, 1102–1587 and 1588–39300, respectively. From each bin, the total number of those corresponding to “Biological process” GO categories of stress response, translation, and ribonucleotide biosynthesis were compared with the average representation for all GO categories containing more than 100 genes (220 categories total).