Fig. 6.
A and B: effects of ANG II, IGF-1, and overexpression of IGF-1R on expression of phospho-(p)Akt (representative experiment). hVSMC were exposed to serum-free medium alone (control) or for 24 h as indicated, and cell lysates were subjected to 10% SDS-PAGE and Western blot analysis with anti-β-actin, anti-total Akt, anti-pAkt, anti-Forkhead rhabdomyosarcoma transcription factor (anti-FKHR), and anti-pFKHR antibodies. C: effect of ANG II on expression of phospho-Akt in quiescent and nonquiescent hVSMC (representative experiment). hVSMC were exposed to serum-free medium alone (control) or with 100 nM ANG II for 24 h, and cell lysates subjected to 10% SDS-PAGE and Western blot analysis.