Figure 8. Hmgb2 is expressed at higher levels in the B6 testis.
(A) Quantitative real-time PCR showing that Hmgb2 but not Tbpl1 transcription is higher in the B6 testis than in the 129 testis. The expression levels are shown as relative levels of the 129 expression which is set as 1. (B) Western blot of Hmgb2 confirming 4 times greater protein in the B6 testis. β-actin is used as loading control. (C) Hmgb2 expression in stage V tubules. Protein levels are higher in the B6 cells, specifically in round spermatids. Scale bar, 50 μm.
