Figure 6. The abnormalities in BrdtΔBD1/ΔBD1 mice are more severe and uniform on the 129 background.
(A-H) H&E staining of histological sections of (A) control129 stage X tubule, (B) control B6 stage X tubule, (C) mutant 129 stage X* tubule, (D) mutant B6 stage X tubule, (E) mutant 129 stage XII* tubule, (F) mutant B6 stage XII tubule, (G) mutant 129 stage V* tubule, and (H) mutant B6 stage V tubule. Grossly normally elongating spermatids are absent in the mutant 129 testes, but predominate the B6 mutant testis. Conversely, compacted but not elongated spermatids are present (C-H, arrowheads and D-G inset) in B6 testes, but they predominate the 129 testes. (see Table 2 for quantification). Spermatids which have neither compacted nor elongating (C, arrow and inset) can be found only in the 129. In 129 testes some spermatids can be found in the spermatocyte layer of the tubules (C,E, and G, open arrows and E inset). Asterisk: although the severe disruption of the order of the seminiferous epithelium in the 129 mutant made precise staging difficult, we refer to approximately staged tubules with a roman numeral followed by an asterisk. Scale bar, 25 μm.