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LuxR-3OC6-HSL stimulation of RNA polymerase binding to the luxI promoter. All lanes contain 6 μM 3OC6-HSL and the same end-labeled probe used in the footprint shown in Fig. 2. Where indicated, LuxR is present at 3.5 nM and E. coli RNA polymerase (RNAP) is present at 3.3 nM. Elements of the luxI promoter are indicated to the right of the gel.
