FIGURE 2.
Inhibition of isoproterenol-induced ATP release from human red blood cells. A, extracellular ATP levels (ATPe) were determined at 20 °C in unstimulated cells (ctrl, empty bar), in cells stimulated 10 min with 3V alone (ctrl, right fine hatched bar), or with 3V preceded by exposure to the following inhibitors: carbenoxolone, 10 μm (CBX 10, left fine hatched bar) or 100 (CBX 100, cross-hatched bar), or 30 μm pannexin 1-selective blocking peptide (10Panx1, right coarse hatched bar) and 30 μm scrambled control peptide (scrPanx1, left coarse hatched bar). Data represent mean ± S.E. of values of quadruplicate determinations from four independent experiments (**, p < 0.01 versus control; ##, p < 0.05 versus 3V control). B, extracellular ATP levels (ATPe) were determined at 37 °C in unstimulated cells (ctrl, empty bar), in cells stimulated 10 min with 3V alone (ctrl, right fine hatched bar), or with 3V preceded by exposure to the following inhibitors: 10 μm carbenoxolone (CBX 10, left fine hatched bar), 30 μm pannexin 1-selective blocking peptide (10Panx1, right coarse hatched bar), or the scrambled control peptide (scrPanx1, left coarse hatched bar). Data represent mean ± S.E. of quadruplicate determinations from three independent experiments (**, p < 0.01 versus control; #, p < 0.01, and ##, p < 0.02 versus 3V control).