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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Cell. 2011 Sep 16;146(6):1029–1041. doi: 10.1016/j.cell.2011.08.016

Table 1. Shotgun bisulfite sequencing of human and chimp sperm methylomes.

Reads: total read ends sequenced (before filtering based on quality). Unique mapped: reads mapping optimally to a single location in the reference genome. Distinct reads: number of genomic locations to which a read maps; when multiple reads map to the same position, one with the best mapping score was selected at random, and all others discarded. Mismatches: average number of mismatches for the reads indicated in the distinct fragments column. Bisulfite conversion rate was calculated at non-CpG cytosines. Percent methylation: proportion of Cs in reads mapping over CpG dinucleotides. Parentheses indicate data from individual donors; sperm without parentheses indicates pooled donors.

Species Sample Mapped Distinct Mis-
matches
BS
conversion
Methylation CpG
coverage
CpGs
covered
Human Sperm (1) 609,127,589 388,835,058 1.58 0.992 0.724 8.8 0.96
Sperm (2) 588,920,777 316,860,245 1.84 0.983 0.674 7.3 0.94
Sperm
(both)
1,198,048,366 705,695,303 1.70 0.988 0.701 16.1 0.96
ESC 940,731,922 366,844,212 0.64 0.988 0.663 14.1 0.93
Chimp Sperm (1) 459,258,834 255,193,493 1.87 0.985 0.665 6.2 0.95
Sperm (2) 520,905,232 327,796,614 1.70 0.984 0.672 7.4 0.94
Sperm
(both)
980,164,066 582,990,107 1.78 0.985 0.669 13.6 0.96