Table 1. Shotgun bisulfite sequencing of human and chimp sperm methylomes.
Reads: total read ends sequenced (before filtering based on quality). Unique mapped: reads mapping optimally to a single location in the reference genome. Distinct reads: number of genomic locations to which a read maps; when multiple reads map to the same position, one with the best mapping score was selected at random, and all others discarded. Mismatches: average number of mismatches for the reads indicated in the distinct fragments column. Bisulfite conversion rate was calculated at non-CpG cytosines. Percent methylation: proportion of Cs in reads mapping over CpG dinucleotides. Parentheses indicate data from individual donors; sperm without parentheses indicates pooled donors.
| Species | Sample | Mapped | Distinct | Mis- matches |
BS conversion |
Methylation | CpG coverage |
CpGs covered |
|---|---|---|---|---|---|---|---|---|
| Human | Sperm (1) | 609,127,589 | 388,835,058 | 1.58 | 0.992 | 0.724 | 8.8 | 0.96 |
| Sperm (2) | 588,920,777 | 316,860,245 | 1.84 | 0.983 | 0.674 | 7.3 | 0.94 | |
|
Sperm
(both) |
1,198,048,366 | 705,695,303 | 1.70 | 0.988 | 0.701 | 16.1 | 0.96 | |
| ESC | 940,731,922 | 366,844,212 | 0.64 | 0.988 | 0.663 | 14.1 | 0.93 | |
| Chimp | Sperm (1) | 459,258,834 | 255,193,493 | 1.87 | 0.985 | 0.665 | 6.2 | 0.95 |
| Sperm (2) | 520,905,232 | 327,796,614 | 1.70 | 0.984 | 0.672 | 7.4 | 0.94 | |
|
Sperm
(both) |
980,164,066 | 582,990,107 | 1.78 | 0.985 | 0.669 | 13.6 | 0.96 |