Table 1.
Gene expression profiling of wtp53 REC-1 MCL cells after exposure to MI-63
| Gene | Fold change | GenBank accession no. | Description |
|---|---|---|---|
| Expression suppressed | |||
| E2F8 | −3.5 | NM_024680 | E2F transcription factor 8 |
| RRM2 | −3.3 | BC001886 | Ribonucleotide reductase M2 polypeptide |
| RRM2 | −3.3 | BE966236 | Ribonucleotide reductase M2 polypeptide |
| CCNE2 | −3.2 | NM_004702 | Cyclin E2 |
| ESCO2 | −2.9 | AL120674 | Establishment of cohesion 1 homolog (Saccharomyces cerevisiae) |
| RAD51AP1 | −2.7 | BE966146 | RAD51-associated protein 1 |
| TK1 | −2.5 | BC007986 | Thymidine kinase 1 soluble |
| CDC6 | −2.5 | U77949 | CDC6 cell division cycle 6 homolog (S cerevisiae) |
| MCM4 | −2.4 | AI859865 | MCM4 minichromosome maintenance deficient 4 (S cerevisiae) |
| SPBC25 | −2.3 | AF225416 | Spindle pole body component 25 homolog (S cerevisiae) |
| Expression induced | |||
| BAX | +3 | NM_004324 | BCL2-associated X protein |
| SESN1 | +3.2 | NM_014454 | Sestrin 1 |
| WIG1 | +3.7 | NM_022470 | p53 target zinc finger protein |
| FAS | +3.7 | Z70519 | FAS (TNF receptor superfamily, member 6) |
| PLK2 | +3.8 | NM_004110 | Polo-like kinase 2 (Drosophila) |
| SULF2 | +3.8 | AL133001 | Sulfatase 2 |
| FAS | +4.3 | AA164751 | FAS (TNF receptor superfamily member 6) |
| FDXR | +4.7 | NM_004110 | Ferreddoxin reductase |
| CDKN1 | +5.1 | NM_000389 | Cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| FAS | +6.5 | NM_000043 | FAS (TNF receptor superfamily member 6) |
REC-1 cells were treated for 24 hours with vehicle or 5μM MI-63. Total cellular RNA was then extracted from triplicate experiments, and gene expression changes were detected using the Affymetrix Human Genome U133 Plus 2.0 array. Fold changes in genes were determined with vehicle- versus MI-63–treated cells using Ingenuity Pathway Analysis software. Ten representative up-regulated and down-regulated genes are shown from the profile.