Figure 3.
Cell survival and BiP/GRP78 gene expression after 16 hours of bortezomib treatment. A: Virtually all cell lines are primary resistant to bortezomib, and only SUDHL-16 cells decrease cell viability up to 25%. B: BiP/GRP78 mRNA expression tends to increase after bortezomib in all cell lines but only reaches statistically significance in SUDHL-6. C: A mild protein induction of BiP/GRP78 was observed only in OCI-LY8 and SUDHL-4 by Western blot, although the differences did not reach statistical significance. Numbers represent the ratio of BiP/GRP78 and α-tubulin optical density. Error bars represent 2 SDs.