FIGURE 3:
Cells expressing an oncogenic, truncated form of APC extrude basally in a cell autonomous manner. (A) Depiction of APC domains. (B) Quantification of cell extrusion events for DLD-1 cells or DLD-1 cells expressing GFP, CT, CTΔPDZ, basic, or EB1-binding domains. p < 0.001 by Student's t test comparing GFP to CT, CTΔPDZ, basic, or EB1-binding domains. (C) Representative images of either apical or basal extrusions from wild-type or APCmcr/mcr zebrafish epidermis (bars, 10 μm). (D) Quantification, where p < 0.001 by Student's t test comparing wild-type to APCmcr/mcr. (E) Quantification of cell autonomous extrusion events of HBE shNS or shAPC cells. p < 0.001 by Student's t test comparing shNS to shAPC. (F) Quantification of cell autonomous extrusion events of DLD-1 GFP or CTΔPDZ cells. p < 0.001 by Student's t test comparing GFP to CTΔPDZ. Error bar, SEM.