Fig. 10.
Effect of endogenous IL-32 on the growth of intracellular M. avium in human macrophages. (A) Wild-type THP-1 cells (open squares), THP-1 cells transfected with a scrambled shRNA (open triangles) or shRNA–IL-32 (clone 7) (closed triangles) were infected with M. avium. Intracellular burden of M. avium was quantified 1 h (0 days), 4 and 8 days after infection. Data represent mean ± SEM of two representative experiments, **P < 0.01, ***P < 0.001. (B) The amount of apoptosis was measured after 24 h of incubation by quantifying percent TUNEL positivity and reported as the percentage of 5000 total cells per condition from three separate experiments (mean ± SEM); *P < 0.05, **P < 0.01. (C) Human MDM cells were infected M. avium with or without 50 ng/ml IL-32γ. The number of cell-associated M. avium was quantified after 1 h (0 day), 2 and 4 days post-infection. Values are mean ± SEM from two independent experiments; *P < 0.05.