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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: Nat Neurosci. 2009 Jul 20;12(8):1020–1027. doi: 10.1038/nn.2371

Figure 3. Evf2 does not affect DLX or MECP2 nuclear localization.

Figure 3

a. Western Blot analysis of DLX2 protein after transfection of a construct containing Dlx2 cDNA and/or Evf2 cDNA. Transfection was performed into C17 mouse neural stem cells in the presence or absence of Evf2 RNA, showing equal levels of DLX2 protein. That Evf2 RNA is stable after transfection was verified by RT-PCR (not shown). b. Western analysis of Evf2TS/TS and wt E13.5 ganglionic eminence extracts probed with anti-DLX2 specific antibody (gift of David Eisentstat) shows that loss of Evf2 does not affect DLX2 protein levels. c–h. Pan-anti-DLX antibody (green) stains nuclei counterstained with DAPI (blue) in both wt and Evf2TS/TS E13.5 MGE. MGE, medial ganglionic eminence; VZ, ventricular zone; SVZ, subventricular zone. c, f: scale bar= 200μm; d, g: scale bar=20μm; e, h: scale bar= 10μm. i–j. Immunohistochemistry using anti-MECP2 antibodies (green) and DAPI (blue nuclei) shows similar nuclear localization in both wt and Evf2TS/TS MGE cells, scale bar=20μm.