Figure 6.
(A–C) Comparison of the catalytic activity of gel purified, anion exchange purified and photocleaved biotin-PC-purified D1235 RNA under native conditions of no heat annealing. (D–F) Comparison of the catalytic activity of gel purified, anion exchange purified and photocleaved biotin-PC-purified D1235 RNA under denaturing conditions of heat annealing and refolding. (G) Exponential fit to the buildup of cleaved substrate product under conditions of no heat annealing using nonlinear least square minimization in SigmaPlot 11.0. (H) Exponential fit to the buildup of cleaved product under conditions of heat annealing. Again error bars are based on standard deviation of 3 experimental repeats.