Figure 2.
Epigenetic profiles of bona fide distal regulatory elements known to be activated via pre-TCR signalling. (A–C) Epigenetic profiles at the Cd8 (A) and Cd4 (B) loci and around the Tcra-associated enhancer Eα (C). In the later case, only the 3′ end of the Tcra locus is shown. The names of the associated enhancers are indicated. Legends are as in Figure 1. (D) Relative gain of H3K4me1 and H3K4me3 at indicated enhancer regions. The average enrichment was quantified by qPCR for each H3K4 methylation mark in ΔRag and ΔRagCD3 thymocytes (Supplementary Figure S3) and the differences between the two cell populations were plotted as a ratio of the signal obtained in ΔRag thymocytes. The relative changes are indicated at the top of each bar.