Figure 2. Leptin-deficiency leads to reduced MMTV-Wnt-1 tumor cell populations and expression of leptin-dependent intracellular signaling protein Akt.
A. Immunofluorescence analysis of tumor cell markers in tumors from WT, ob/ob, and db/db mice. Formaldehyde fixed tissues were sectioned and immunostained for expression of cellular markers keratin 6, 8, and 14. Representative images shown (Bar = 100 µm). Inset shows magnified view of acini in keratin 14 panels. B. Quantification of keratin 6 immunoreactive cells in sections of tumors from WT, ob/ob, db/db mice. Data are representative of an analysis repeated at least three times and presented as mean ± SEM. C. Immunofluorescence analysis of total and phosphorylated Akt in WT, ob/ob, and db/db mice. D. Quantification of p-Akt immunopositive cells in tumors from WT, ob/ob, and db/db mice. Data are representative of an analysis repeated at least three times and presented as mean ± SEM. Comparisons between the ob/ob and WT mice were performed on equivalent sized tumors.