. 2011 Aug 19;301(4):H1506–H1512. doi: 10.1152/ajpheart.00587.2011

Table 1.

Densitometry of Western blots from Figs. 2, A and B, and 3

	WT	SIRT1^+/−	SIRT1⁺⁺⁺
Figure 2, A and B
Lysine acetylation/protein
Homogenate	0.26 ± 0.01	0.35 ± 0.005	0.20 ± 0.01^*
Nucleus	0.18 ± 0.04	0.21 ± 0.05	0.19 ± 0.05
Mitochondria	0.49 ± 0.09	0.55 ± 0.06	0.54 ± 0.10
Cytosol	0.48 ± 0.01^*	0.71 ± 0.03^†	0.31 ± 0.01^*^†
Figure 3
SIRT1/actin	1.02 ± 0.18	0.54 ± 0.05	2.08 ± 0.34^*
Phospho-eNOS/eNOS	0.67 ± 0.11	0.44 ± 0.05	1.58 ± 0.17^*
Ac p65/p65	0.49 ± 0.02^*	0.64 ± 0.02	0.49 ± 0.01^*
MnSOD/actin	1.38 ± 0.05	1.36 ± 0.09	1.23± 0.07
LC3 II/I	2.78 ± 0.64	3.59 ± 0.50	4.44 ± 1.20

Data are means ± SE; n ≥ 3. WT, wild type; SIRT1^+/−, silent information regulator of transcription 1 (SIRT1) deficient; SIRT1⁺⁺⁺, SIRT overexpressing. For Fig. 2, A and B, lysine acetylation densitometry was performed in the range of 25–100 kDa and normalized to protein across the same molecular mass range from Ponceau S-stained membranes. For Fig. 3, SIRT1 and MnSOD were normalized to actin; phospho-endothelial nitric oxide synthase (eNOS), Ac p65, and LC3 II were normalized to eNOS, p65, and LC3 I, respectively.

P < 0.05 vs. SIRT1^+/−;

^†

P < 0.05 vs. WT (by ANOVA).