Table 1.
Comparison of traditional gating and SPADE, expressed in terms of the number of overlapping cells between each traditionally-derived gate and each annotated SPADE region. The total number of cells in each gate and each SPADE region are listed. The percentages are defined as the ratio between the number of overlapping cells and the total number of cells in the corresponding gate, thereby representing the percent of cells in a gate that are assigned to each SPADE region. Large values in shaded entries indicate the consistency between traditional gating and SPADE.
| Gates in the gating analysis | |||||||
|---|---|---|---|---|---|---|---|
| B cell - 150314 | T cell - 14699 | CD4+ - 2808 | CD8+ - 6055 | myeloid - 209079 | HSPC - 418 | ||
| Annotated SPADE branches | B cell - 152685 | 146017 (97.1%) | 88 (0.6%) | 0 | 0 | 2246 (1.1%) | 16 (3.8%) |
| Dendritic - 3996 | 3562 (2.4%) | 79 (0.5%) | 77 (2.7%) | 0 | 83 (<0.1%) | 0 | |
| T cell – 17538 | 364 (0.2%) | 12377 (84.2%) | 2729 (97.2%) | 6037 (99.7%) | 3033 (1.5%) | 0 | |
| CD4+ - 2931 | 0 | 2858 (19.4%) | 2713 (96.6%) | 0 | 27 (<0.1%) | 0 | |
| CD8+ - 6301 | 0 | 6174 (42%) | 0 | 5843 (96.5%) | 32 (<0.1%) | 0 | |
| myeloid - 202180 | 5 (<0.1%) | 75 (0.5%) | 0 | 0 | 199048 (95.2%) | 0 | |
| c-kit+ - 15681 | 8 (<0.1%) | 815 (5.5%) | 2 (0.1%) | 9 (<0.1%) | 1159 (0.6%) | 401 (95.9%) | |