Figure 3. HIV-specific production of IL-10 and IL-2 by CD4+CD25high T cells.
A) Frequency of CD4+CD25− and CD4+CD25high producing IL-2 (left) or IL-10 (right) following either polyclonal or p24 stimulation. Cells (5.104/well) were incubated for 24 hours alone or in presence of either 5μg/ml plate bound anti-CD3 mAb and soluble anti-CD28 or of p24 antigen (5 μg/ml) and 5.103 monocytes/well, before assessment of intracellular production of cytokines by flow cytometry. Changes from baseline in the mean frequencies of cells producing cytokines following stimulation are indicated. Results from 6 patients studied are presented. B) Quantitative RT-PCR analyses of Foxp3 (left panel), IL-2 (middle) and IL-10 (right) expression of CD4+CD25− and CD4+CD25high after purification and non-stimulated (NS), or stimulated either with anti-CD3 and anti-CD28 antibodies or p24 antigen. Mean from 5 patients are presented. Ct values were normalized to mRNA for SF3A1 expression. (* P< 0.05; ** P< 0.01, Mann Whitney rank sum test).