Figure 2.
FOXA2 knockdown induces EMT and promote cell migration of A549 cells. (A) The effect of FOXA2 knockdown on EMT was examined by observing the morphology (left bottom) and measuring the expression levels of ZO-1, E-cadherin, and fibronectin by western blotting (left top) and immunofluorescence staining (middle and right). (B) Increased cell migration caused by knockdown of FOXA2 was determined by transwell assay. A reprentative result of three independent (left) and statistic data were presented. (C) At 8 weeks after tail vein injection of sramble or shFOXA2-transfected A549 cells, metastasis and/or tumor formation in lung was determined under a dissection microscope (left top) and by histological analysis using H and E (hematoxylin and eosin) staining (left bottom). Right panel, the relative concentration of circulating tumor cells in blood was determined by qRT-PCR. (D) 3H-thymidine incorporation analysis was performed to determine the effect of FOXA2 knockdown on the proliferation rate of cells.