Fig. 1.
Identification of the erythrocyte binding region of Py235. (A) Schematic representation of three members of the RH multigene family (PfRH1, PfR4, and Py235). The location of the erythrocyte binding region (EBD) or nucleotide binding domain (NBD) and that the transmembrane domain (TM) are indicated. The amino acid numbers for the beginning and end of the EBD and NBD are indicated. (B) Effect of enzyme treatment on erythrocyte binding of the entire Py235 protein. Five micrograms of Py235 was incubated with untreated (Un), neuraminidase-treated (Ne), chymotrypsin-treated (Chymo), or trypsin-treated (Tryp) mouse erythrocytes. Purified Py235 (left) was detected by anti-His antibody.