FIGURE 2.
WWP1 repressed senescence-associated features in 2BS and WI38 cells. The stable transformants were passaged until senescence was achieved. They were then analyzed for the related senescence markers. A, Western blot analysis of WWP1 expression levels in WWP1-infected or WWP1 shRNA-infected cells. B, WWP1-transfected and WWP1 shRNA-transfected 2BS and WI38 cells were stained for SA-β-gal activity (upper panel) and formation of SAHF (middle and lower panels). C, growth curves of WWP1-transfected and WWP1 shRNA-transfected cells were determined by the MTT assay. Values are the mean ± S.D. of triplicate points from a representative experiment (n = 3), which was repeated three times with similar results. D, flow cytometry analysis of WWP1-transfected and WWP1 shRNA-transfected 2BS cells. Values represent the means ± S.E. of triplicate points from a representative experiment (n = 3), which was repeated three times. sh, shRNA.