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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: Mol Cell. 2011 Oct 7;44(1):134–146. doi: 10.1016/j.molcel.2011.06.038

Figure 6. p62 regulates mTOR translocation to the lysosomal compartment.

Figure 6

Starved or restimulated 293 cells were co-immunostained for p62 and (A) LAMP2, (B) RagC, (C) mTOR, or (D) raptor. (E) p62fl/fl MEFs infected with GFP or CRE adenovirus and overexpressing activated RagB were starved for 4 h and then restimulated with amino acids for 10 min. Afterwards, they were co-immunostained for mTOR and LAMP2. (F) Images of WT and p62 KO immortal MEFs co-immunostained for RagC and LAMP2. In all images, inserts show selected fields that were magnified two or five times to show areas of staining overlap (Merge). Scale bars=10 µm. Images are representative of three experiments.