Fig. 4.
Cleavage of Nup62 by 2Apro. (A) Recombinant GST-Nup62 (1 nmol) was incubated with the indicated recombinant 2Apro (0.2 nmol) as described in Materials and Methods. The samples were fractionated by electrophoresis and then visualized by Coomassie staining. Cleavage product identities, based on molecular weight (GST plus Nup62), are approximated according to sites mapped by Park et al. (30). “N” indicates that the cleavage product is the amino fragment from Nup62 cleavage at the designated site, and “C” indicates the carboxy fragment. (B) Gels similar to those in panel A recorded substrate loss over a time course of 8 h. The densitometry results for the substrate bands relative to control lanes were plotted.