Figure 6.
N- or C-terminal deletions of c-Myc do not affect its ability to repress the p21 promoter. CAT assays were performed with extracts from Caco-2 cells transfected with the empty vector or with expression plasmids encoding the c-Myc deletion mutants c-MycΔ41–178 or c-MycΔ352–439 and the p21 promoter–reporter construct CAT119. The amount of extract that was loaded for each CAT assay was normalized for expression of the β-galactosidase control. All transfections were repeated at least three times in duplicate. Graphs represent the results of three independent experiments, with bars indicating SD.