Figure 1. PKC downregulation of IKs requires functional dynamin.
A. Exemplar traces showing currents recorded in CHO cells co-transfected with cDNA encoding KCNQ1 and KCNE1, untreated or after treatment with PMA (50 nM, 30 minutes), as indicated. Voltage protocol inset.
B. Mean peak current density from CHO cells transfected with cDNA encoding KCNQ1 and KCNE1 untreated (n = 73) or after treatment with PMA (n = 25); symbols as in panel A. ** p < 0.001.
C. Exemplar traces showing currents recorded in CHO cells co-transfected with cDNA encoding KCNQ1, KCNE1, and either wild-type dynamin 2 or K44A dynamin 2, untreated or after treatment with PMA (50 nM), as indicated. Voltage protocol inset.
D. Mean peak current density from CHO cells co-transfected with cDNA encoding KCNQ1, KCNE1, and wild-type dynamin 2, untreated (n = 18) or after treatment with PMA (n = 6); or KCNQ1, KCNE1, and K44A dynamin 2, untreated (n = 20) or after treatment with PMA (n = 6); symbols as in panel C. * p < 0.05; ** p < 0.001.
E. Time-to-half-peak current at +60 mV for cells co-expressing KCNQ1 and KCNE1, alone or with either wild-type or K44A dynamin 2, with or without PMA treatment, as indicated; n values as for panel D. * p < 0.05; ** p < 0.001.