Fig. 2.
Enlarged dentate gyrus (DG) in young ApoE-deficient mice. (A) DG volume quantification over time using three-dimensional volumetric reconstructions following Nissl staining. DG volume is significantly enlarged in ApoE-deficient mice (ApoE KO) compared with wild type (WT) at 1 and 2 months of age. (B,C) The DG granular layer at 1 month of age is enlarged in ApoE deficiency (C) compared with wild type (B). (D) Quantification of NeuN-positive mature neurons shows that there are more NeuN-positive neurons in ApoE deficient DG compared with wild-type DG. (E,F) The enlargement in the 1-month-old DG is due to increased cell number in ApoE deficiency (F) compared with wild type (E), as shown by NeuN staining. Data are derived from four animals per group (except the 4-month-old ApoE-deficient group in A, for which there are three animals). Error bars represent s.e.m. *P<0.05, **P<0.01. Scale bars: 200 μm in B; 50 μm in E.