Table 1. Predicted HLA-A2 affinity and homology of Human and Mouse SSX-2 nonamer peptides.
Shown are the peptide names, amino acid sequences, and predicted HLA-A2 affinity scores of potential HLA-A2 peptides derived from the amino acid sequence of human SSX-2, as well as the respective murine Ssxa1 and Ssxb1 peptide sequences and binding affinities. Predicted HLA-A2 peptide affinity was determined by scanning the SSX-2, Ssxa1, and Ssxb1 protein sequence with two different peptide prediction algorithms [29, 30]. A known HLA-A2-binding peptide derived from the influenza A matrix protein is shown for comparison [39]. Amino acids within the mouse peptide sequences shown in gray represent identical peptide residues between the human and murine peptides.
| Human SSX-2 Peptide Name | Peptide Sequence | Predicted Binding Affinity [BIMAS] | Predicted Binding Affinity [SYFPEITHI] | Mouse Peptide Homologue (Ssxa1 Ssxb1) | Predicted Binding Affinity [BIMAS] | Predicted Binding Affinity [SYFPEITHI] |
|---|---|---|---|---|---|---|
| SSX2p16 | QIPEKIQKA | 6.4 | 21 |
|
0.001 | 8 |
| 1.022 | 12 | |||||
|
| ||||||
| SSX2p20 | KIQKAFDDI | 4.7 | 17 |
|
0.010 | 9 |
| 2.726 | 16 | |||||
|
| ||||||
| SSX2p41 | KASEKIFYV | 1017.1 | 22 |
|
0.501 | 19 |
| 321.9 | 14 | |||||
|
| ||||||
| SSX2p57 | AMTKLGFKA | 20.8 | 16 |
|
3.588 | 13 |
| 205.9 | 23 | |||||
|
| ||||||
| SSX2p99 | MTFGRLQGI | 2.1 | 20 | Deleted | - | - |
|
| ||||||
| SSX2p103 | RLQGISPKI | 10.4 | 23 | Deleted | - | - |
|
| ||||||
| SSX2p167 | RLRERKQLV | 21.7 | 22 |
|
21.672 | 22 |
| 0.029 | 19 | |||||
|
| ||||||
| Influenza | GILGFVFTL | 550.9 | 30 | N/A | - | - |