Figure 3.
RecBCD-pathway recombination of λ in the absence of RuvC and RecG. The three graphs summarize the results of three different experimental designs, measuring the efficiency of λ recombination in rec+ and ruvC recG cells. For each design, we used a different set of phages: nin+ (left), Δnin (middle), and Δnin Chi+/o (right). Each bar represents the mean percentage of homologous recombination among site-specific Int-mediated recombinants (±s.d.; calculated as described in Fig. 2). Three independent experiments were performed for nin+ and Δnin crosses. Two experiments were performed for the Δnin Chi+/o cross. The deletion Δnin shortens the DNA segment whose recombination is assayed (see Fig. 2) and therefore necessarily decreases the percent recombination relative to nin+ crosses. Thus, the important comparison for both nin+ and Δnin crosses is between presence or absence of RuvC RecG in each.