Figure 6.
HCl treatment is disruptive to nuclear structure. (A) Top panels compare sites of BrdU incorporation (Harlan/Sera-Lab, green) to localization p150 (CAF-1; red) with increasing acid concentration. No DNaseI is used in this experiment. At concentrations <1 N HCl, BrdU is not sufficiently exposed for detection. Lower panels depict nuclear lamins A/C localization (red) in increasing HCl concentration. (B) Cells were stained for BrdU with DNaseI and then exposed to HCl. BrdU incorporation sites (green, Becton Dickinson) are compared to the nucleolus as defined by staining with a human nucleolar autoantibody (red, Sigma; C).