Figure 2.
IRF-3a is generated by alternative splicing. (A) Chromosomal location of the IRF-3a-specific sequence. Open boxes correspond to exon assignments (Lowther et al. 1999). Exonic sequences are capitalized; splice donor and acceptor sites are bolded and underlined. (B) Schematic of the exon–intron structure of the 5′ part of the IRF-3 gene and of the IRF-3 and IRF-3a-specific splicing patterns. (C) RNase protection analysis of the IRF-3a-specific splicing pattern. PCR-amplified fragment of IRF-3a cDNA was used to generate an antisense riboprobe, which was hybridized with the total RNA from a panel of cell types. Lines represent vector sequences remaining in the probe. The numbers correspond to the sizes of the respective fragments. Schematic to the right of the blot shows the inferred structure of the protected fragments.