Figure 6. Evolutionary conservation of mutation enhancer elements.
(A) The predicted location of the 5′MEE and 3′MEE (red bars) as determined by three way comparison between chicken (Genbank NW_001471461.1, nt 1149180–1151691 and nt 1148230–1148400), zebra finch (Genbank NW_002197395.1, nt 5112960–5107578 and nt 5106902–5107106), and California condor (GenBank JF693631, nt 1–800, and GenBank HQ414233, nt 478–680) using MULAN. Evolutionarily conserved transcription factor binding sites predicted by multiTF are shown in blue binding sites, and a highly conserved sequence motif that might play a role in MEE function is highlighted in orange. A chicken IGL silencer element annotated in GenBank L26587 is shown as a black line. (B) Location of the evolutionarily conserved sequence element with respect to the FragB and FragC clones (see Fig. 2) which show equal transcription but are deficient or active for SHM/GCV, respectively.