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NPAT also activates transcription from histone H2B and H3 promoters. U2OS cells were transfected with 1 μg pCMV (vector) or 1 μg pCMV–NPAT together with 50 ng pGLH2B (labeled as H2B), pGLH2B(65) [labeled as H2B(65)], pGLH2B(65M) [labeled as H2B(65M)], or pGLH3 (labeled as H3) reporter construct. 50 ng pCMV–lacZ was also cotransfected in each transfection. Twenty-two hours after transfection, cells were lysed and the fold of induction was measured as described in Fig. 6A.
