Figure 7.
Co-localization of cyclin E with anti-phospho-T1270 reactive foci in a cell cycle–dependent manner. (a) Cyclin E (red) co-localizes with p220 (green) in a cell containing four p220 foci. Nuclei are in blue. (b–e) Growing diploid fibroblasts were labeled with BrdU for 1 h and subjected to immunofluorescence using anti-phospho-T1270 (red), anti-cyclin E (green), anti-BrdU (magenta), and 4′,6-diamidino-2-phenylindole (DAPI; blue) to visualize nuclei. (b) A BrdU-negative cell containing two phospho-T1270 foci that co-localize with cyclin E. This field also contains a BrdU-negative cell that is negative for both anti–cyclin E and anti-phospho-T1270 antibodies. (c) A BrdU-positive cell containing two phospho-T1270 foci that co-localize with cyclin E adjacent to a BrdU-negative cell that is negative for both anti–cyclin E and anti-phospho-T1270 antibodies. (d) BrdU-positive cells containing two or four phospho-T1270 foci that co-localize with cyclin E. (e) A BrdU-negative cell containing four phospho-T1270 foci that lacks cyclin E staining.