Figure 7.
Cross-linking of the α subunit to the DNA substrate (T4G4)2. (A) To monitor complex formation, the DNA was 5′-radiolabeled, incubated with the α subunit, and cross-linked. Cross-linked complexes were monitored by denaturing polyacrylamide gel electrophoresis. (B) To evaluate whether the α–DNA and α–DNA–α cross-linked complexes were extended by telomerase, increasing concentrations of the α subunit were cross-linked to nonlabeled DNA. 32P-Radiolabeled nucleotides and nuclear extract were added, and the label incorporation into the complexes was monitored by gel electrophoresis.