Fig. 4.
Foxp3+ T cells in foreign-pMHCII–specific populations. (A) Representative dot plot of Foxp3 and Helios expression in CD4+ and 2W:I-Ab+CD4+ T cells following enrichment of total lymph node and spleen cells from naive B6 mice. (B) Representative contour plots of CD4+ T cells following enrichment of total lymph node and spleen cells from naive Foxp3GFP mice with the indicated tetramers. (C) Scatter plot displaying percentages of Foxp3+ cells within each pMHCII-specific T-cell population in individual animals, with mean values indicated. (D) Percentages and absolute numbers of Foxp3+ and Foxp3− 2W:I-Ab+ T cells from WT Tcra+/− or Act-2W Tcra+/− mice. Mean values, P values, and limits of detection are indicated.